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rabbit anti lag3  (Novus Biologicals)


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    Novus Biologicals rabbit anti lag3
    (A) Immunofluorescent histochemistry analyses of tibia sections from uninfected and MRSA-infected humanized BLT mice 14 days post-op were performed with labeled antibodies against CD3, LAG-3, TIM-3, and PD-1 with DAPI counter stain, and representative images are shown at 4x. Note the increased numbers of T cells near the SAC (dashed yellow line) in the infected tibiae. (B) A multichromatic spectral flow cytometry analyses were performed on tibial bone marrow cells from uninfected and MRSA-infected BLT mice. Live human CD45+/CD3+/T cells and their subpopulations (CD4+, CD8+, Tregs) were analysed for immune checkpoint expression <t>(LAG3,</t> TIM-3, and PD-1) and proliferation (Ki67), and representative histograms are shown. Note the frequency of human CD3 + /CD4 + T cells expressing TIM-3, LAG3 & PD-1) in the cells from MRSA-infected bone marrow (n=4-8 mice, *p<0.05, t-test).
    Rabbit Anti Lag3, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti lag3/product/Novus Biologicals
    Average 93 stars, based on 5 article reviews
    rabbit anti lag3 - by Bioz Stars, 2026-02
    93/100 stars

    Images

    1) Product Images from "Immune Checkpoint Molecules as Biomarkers of Staphylococcus aureus Bone Infection and Clinical Outcome"

    Article Title: Immune Checkpoint Molecules as Biomarkers of Staphylococcus aureus Bone Infection and Clinical Outcome

    Journal: bioRxiv

    doi: 10.1101/2024.12.30.630837

    (A) Immunofluorescent histochemistry analyses of tibia sections from uninfected and MRSA-infected humanized BLT mice 14 days post-op were performed with labeled antibodies against CD3, LAG-3, TIM-3, and PD-1 with DAPI counter stain, and representative images are shown at 4x. Note the increased numbers of T cells near the SAC (dashed yellow line) in the infected tibiae. (B) A multichromatic spectral flow cytometry analyses were performed on tibial bone marrow cells from uninfected and MRSA-infected BLT mice. Live human CD45+/CD3+/T cells and their subpopulations (CD4+, CD8+, Tregs) were analysed for immune checkpoint expression (LAG3, TIM-3, and PD-1) and proliferation (Ki67), and representative histograms are shown. Note the frequency of human CD3 + /CD4 + T cells expressing TIM-3, LAG3 & PD-1) in the cells from MRSA-infected bone marrow (n=4-8 mice, *p<0.05, t-test).
    Figure Legend Snippet: (A) Immunofluorescent histochemistry analyses of tibia sections from uninfected and MRSA-infected humanized BLT mice 14 days post-op were performed with labeled antibodies against CD3, LAG-3, TIM-3, and PD-1 with DAPI counter stain, and representative images are shown at 4x. Note the increased numbers of T cells near the SAC (dashed yellow line) in the infected tibiae. (B) A multichromatic spectral flow cytometry analyses were performed on tibial bone marrow cells from uninfected and MRSA-infected BLT mice. Live human CD45+/CD3+/T cells and their subpopulations (CD4+, CD8+, Tregs) were analysed for immune checkpoint expression (LAG3, TIM-3, and PD-1) and proliferation (Ki67), and representative histograms are shown. Note the frequency of human CD3 + /CD4 + T cells expressing TIM-3, LAG3 & PD-1) in the cells from MRSA-infected bone marrow (n=4-8 mice, *p<0.05, t-test).

    Techniques Used: Infection, Labeling, Staining, Flow Cytometry, Expressing



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    (A) Immunofluorescent histochemistry analyses of tibia sections from uninfected and MRSA-infected humanized BLT mice 14 days post-op were performed with labeled antibodies against CD3, LAG-3, TIM-3, and PD-1 with DAPI counter stain, and representative images are shown at 4x. Note the increased numbers of T cells near the SAC (dashed yellow line) in the infected tibiae. (B) A multichromatic spectral flow cytometry analyses were performed on tibial bone marrow cells from uninfected and MRSA-infected BLT mice. Live human CD45+/CD3+/T cells and their subpopulations (CD4+, CD8+, Tregs) were analysed for immune checkpoint expression <t>(LAG3,</t> TIM-3, and PD-1) and proliferation (Ki67), and representative histograms are shown. Note the frequency of human CD3 + /CD4 + T cells expressing TIM-3, LAG3 & PD-1) in the cells from MRSA-infected bone marrow (n=4-8 mice, *p<0.05, t-test).
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    (A) Immunofluorescent histochemistry analyses of tibia sections from uninfected and MRSA-infected humanized BLT mice 14 days post-op were performed with labeled antibodies against CD3, LAG-3, TIM-3, and PD-1 with DAPI counter stain, and representative images are shown at 4x. Note the increased numbers of T cells near the SAC (dashed yellow line) in the infected tibiae. (B) A multichromatic spectral flow cytometry analyses were performed on tibial bone marrow cells from uninfected and MRSA-infected BLT mice. Live human CD45+/CD3+/T cells and their subpopulations (CD4+, CD8+, Tregs) were analysed for immune checkpoint expression <t>(LAG3,</t> TIM-3, and PD-1) and proliferation (Ki67), and representative histograms are shown. Note the frequency of human CD3 + /CD4 + T cells expressing TIM-3, LAG3 & PD-1) in the cells from MRSA-infected bone marrow (n=4-8 mice, *p<0.05, t-test).
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    (A) Immunofluorescent histochemistry analyses of tibia sections from uninfected and MRSA-infected humanized BLT mice 14 days post-op were performed with labeled antibodies against CD3, LAG-3, TIM-3, and PD-1 with DAPI counter stain, and representative images are shown at 4x. Note the increased numbers of T cells near the SAC (dashed yellow line) in the infected tibiae. (B) A multichromatic spectral flow cytometry analyses were performed on tibial bone marrow cells from uninfected and MRSA-infected BLT mice. Live human CD45+/CD3+/T cells and their subpopulations (CD4+, CD8+, Tregs) were analysed for immune checkpoint expression <t>(LAG3,</t> TIM-3, and PD-1) and proliferation (Ki67), and representative histograms are shown. Note the frequency of human CD3 + /CD4 + T cells expressing TIM-3, LAG3 & PD-1) in the cells from MRSA-infected bone marrow (n=4-8 mice, *p<0.05, t-test).
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    (A) Immunofluorescent histochemistry analyses of tibia sections from uninfected and MRSA-infected humanized BLT mice 14 days post-op were performed with labeled antibodies against CD3, LAG-3, TIM-3, and PD-1 with DAPI counter stain, and representative images are shown at 4x. Note the increased numbers of T cells near the SAC (dashed yellow line) in the infected tibiae. (B) A multichromatic spectral flow cytometry analyses were performed on tibial bone marrow cells from uninfected and MRSA-infected BLT mice. Live human CD45+/CD3+/T cells and their subpopulations (CD4+, CD8+, Tregs) were analysed for immune checkpoint expression <t>(LAG3,</t> TIM-3, and PD-1) and proliferation (Ki67), and representative histograms are shown. Note the frequency of human CD3 + /CD4 + T cells expressing TIM-3, LAG3 & PD-1) in the cells from MRSA-infected bone marrow (n=4-8 mice, *p<0.05, t-test).
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    (A) Immunofluorescent histochemistry analyses of tibia sections from uninfected and MRSA-infected humanized BLT mice 14 days post-op were performed with labeled antibodies against CD3, LAG-3, TIM-3, and PD-1 with DAPI counter stain, and representative images are shown at 4x. Note the increased numbers of T cells near the SAC (dashed yellow line) in the infected tibiae. (B) A multichromatic spectral flow cytometry analyses were performed on tibial bone marrow cells from uninfected and MRSA-infected BLT mice. Live human CD45+/CD3+/T cells and their subpopulations (CD4+, CD8+, Tregs) were analysed for immune checkpoint expression <t>(LAG3,</t> TIM-3, and PD-1) and proliferation (Ki67), and representative histograms are shown. Note the frequency of human CD3 + /CD4 + T cells expressing TIM-3, LAG3 & PD-1) in the cells from MRSA-infected bone marrow (n=4-8 mice, *p<0.05, t-test).
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    Representative immunoreactivity patterns of TILs and immune checkpoint expression in gastric cancer tissues: (A) GC immune environment using multiplex fluorescence IHC (mIHC) panels scanned using Vectra software. A1: lymphocyte panel of CD3, CD4, and CD8 with CK staining. A2: B lymphocyte panel of CD20 with CK staining. A3: myeloid panel of CD66B and CD68 with CK staining. A4: immune therapy target panel of PD-1 and <t>LAG3</t> with CK staining (green: CK; blue: DAPI; left column magnification ×20; right column magnification ×400). (B) Quantified protein expression using the immunohistochemistry (IHC) score (intensity: brown, intense staining; orange, moderate staining; yellow, weak staining; and blue, no staining. Left column magnification ×20; right column magnification ×400). B1: CTLA4 in cancer tissues. B2: CTLA4 expression in cancer cells was scored using software. B3: CTLA4 expression in TILs was scored using software. B4: PD-L1 in cancer tissues. B5: PD-L1 expression in cancer cells was scored using software. B6: PD-L1 expression in TILs was scored using software.
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    Image Search Results


    (A) Immunofluorescent histochemistry analyses of tibia sections from uninfected and MRSA-infected humanized BLT mice 14 days post-op were performed with labeled antibodies against CD3, LAG-3, TIM-3, and PD-1 with DAPI counter stain, and representative images are shown at 4x. Note the increased numbers of T cells near the SAC (dashed yellow line) in the infected tibiae. (B) A multichromatic spectral flow cytometry analyses were performed on tibial bone marrow cells from uninfected and MRSA-infected BLT mice. Live human CD45+/CD3+/T cells and their subpopulations (CD4+, CD8+, Tregs) were analysed for immune checkpoint expression (LAG3, TIM-3, and PD-1) and proliferation (Ki67), and representative histograms are shown. Note the frequency of human CD3 + /CD4 + T cells expressing TIM-3, LAG3 & PD-1) in the cells from MRSA-infected bone marrow (n=4-8 mice, *p<0.05, t-test).

    Journal: bioRxiv

    Article Title: Immune Checkpoint Molecules as Biomarkers of Staphylococcus aureus Bone Infection and Clinical Outcome

    doi: 10.1101/2024.12.30.630837

    Figure Lengend Snippet: (A) Immunofluorescent histochemistry analyses of tibia sections from uninfected and MRSA-infected humanized BLT mice 14 days post-op were performed with labeled antibodies against CD3, LAG-3, TIM-3, and PD-1 with DAPI counter stain, and representative images are shown at 4x. Note the increased numbers of T cells near the SAC (dashed yellow line) in the infected tibiae. (B) A multichromatic spectral flow cytometry analyses were performed on tibial bone marrow cells from uninfected and MRSA-infected BLT mice. Live human CD45+/CD3+/T cells and their subpopulations (CD4+, CD8+, Tregs) were analysed for immune checkpoint expression (LAG3, TIM-3, and PD-1) and proliferation (Ki67), and representative histograms are shown. Note the frequency of human CD3 + /CD4 + T cells expressing TIM-3, LAG3 & PD-1) in the cells from MRSA-infected bone marrow (n=4-8 mice, *p<0.05, t-test).

    Article Snippet: Primary antibodies: The following antibodies were utilized for immunostaining: goat anti-CD3ε (clone M-20, sc-1127, RRID:AB_631128, Santa Cruz Biotechnology), mouse anti-PD-1 (10377-MM23, RRID:AB_2936309, Sino Biologicals), Rabbit anti-LAG3 (clone BLR027F, NBP2-76402, RRID:AB_3403543, Novus Biologicals), Mouse anti-TIM3/HAVCR2 (clone TIM3/4031, V8754-20UG, NSJ Bioreagents), Rabbit anti- S. aureus (PA1-7246, RRID:AB_561546, Thermo Fisher Scientific), and Mouse anti-CD66b (G10F5, NBP2-80664, RRID:AB_3096017, Novus Biologicals).

    Techniques: Infection, Labeling, Staining, Flow Cytometry, Expressing

    Representative immunoreactivity patterns of TILs and immune checkpoint expression in gastric cancer tissues: (A) GC immune environment using multiplex fluorescence IHC (mIHC) panels scanned using Vectra software. A1: lymphocyte panel of CD3, CD4, and CD8 with CK staining. A2: B lymphocyte panel of CD20 with CK staining. A3: myeloid panel of CD66B and CD68 with CK staining. A4: immune therapy target panel of PD-1 and LAG3 with CK staining (green: CK; blue: DAPI; left column magnification ×20; right column magnification ×400). (B) Quantified protein expression using the immunohistochemistry (IHC) score (intensity: brown, intense staining; orange, moderate staining; yellow, weak staining; and blue, no staining. Left column magnification ×20; right column magnification ×400). B1: CTLA4 in cancer tissues. B2: CTLA4 expression in cancer cells was scored using software. B3: CTLA4 expression in TILs was scored using software. B4: PD-L1 in cancer tissues. B5: PD-L1 expression in cancer cells was scored using software. B6: PD-L1 expression in TILs was scored using software.

    Journal: Frontiers in Pharmacology

    Article Title: Chemokine ligand 14 correlates with immune cell infiltration in the gastric cancer microenvironment in predicting unfavorable prognosis

    doi: 10.3389/fphar.2024.1397656

    Figure Lengend Snippet: Representative immunoreactivity patterns of TILs and immune checkpoint expression in gastric cancer tissues: (A) GC immune environment using multiplex fluorescence IHC (mIHC) panels scanned using Vectra software. A1: lymphocyte panel of CD3, CD4, and CD8 with CK staining. A2: B lymphocyte panel of CD20 with CK staining. A3: myeloid panel of CD66B and CD68 with CK staining. A4: immune therapy target panel of PD-1 and LAG3 with CK staining (green: CK; blue: DAPI; left column magnification ×20; right column magnification ×400). (B) Quantified protein expression using the immunohistochemistry (IHC) score (intensity: brown, intense staining; orange, moderate staining; yellow, weak staining; and blue, no staining. Left column magnification ×20; right column magnification ×400). B1: CTLA4 in cancer tissues. B2: CTLA4 expression in cancer cells was scored using software. B3: CTLA4 expression in TILs was scored using software. B4: PD-L1 in cancer tissues. B5: PD-L1 expression in cancer cells was scored using software. B6: PD-L1 expression in TILs was scored using software.

    Article Snippet: The following major antibodies were utilized: anti-CCL14 antibody (1:50; sc-376152, santa, United States), anti-CD3 antibody (1:200; 85061, CST, United States), anti-CD4 antibody (1:200; ab133616, Abcam, United Kingdom), anti-CD8 antibody (ab93278, Abcam), anti-CD66b (1:500; arg66287, Airgobio, China), anti-CD68 antibody (1:400;76437, CST, United States), anti-CTLA4 antibody (1:50; NB100-64849, Novus, United States), anti-LAG3 antibody (1:50; 15372, CST, United States), anti-PD-1 antibody (1:50; 86163, CST, United States), anti-PD-L1 antibody (1:50; CST, United States), and anti-cytokeratin antibody (1:400; orb69073, Biorbyt, United Kingdom).

    Techniques: Expressing, Multiplex Assay, Fluorescence, Software, Staining, Immunohistochemistry

    Correlation between CCL14 expression and tumor-infiltrating immune cells (TILs) and immune checkpoints.

    Journal: Frontiers in Pharmacology

    Article Title: Chemokine ligand 14 correlates with immune cell infiltration in the gastric cancer microenvironment in predicting unfavorable prognosis

    doi: 10.3389/fphar.2024.1397656

    Figure Lengend Snippet: Correlation between CCL14 expression and tumor-infiltrating immune cells (TILs) and immune checkpoints.

    Article Snippet: The following major antibodies were utilized: anti-CCL14 antibody (1:50; sc-376152, santa, United States), anti-CD3 antibody (1:200; 85061, CST, United States), anti-CD4 antibody (1:200; ab133616, Abcam, United Kingdom), anti-CD8 antibody (ab93278, Abcam), anti-CD66b (1:500; arg66287, Airgobio, China), anti-CD68 antibody (1:400;76437, CST, United States), anti-CTLA4 antibody (1:50; NB100-64849, Novus, United States), anti-LAG3 antibody (1:50; 15372, CST, United States), anti-PD-1 antibody (1:50; 86163, CST, United States), anti-PD-L1 antibody (1:50; CST, United States), and anti-cytokeratin antibody (1:400; orb69073, Biorbyt, United Kingdom).

    Techniques: Expressing